Part:BBa_K5452133:Design

RISC casette

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 6555
  Illegal PstI site found at 1946
  Illegal PstI site found at 4982
  Illegal PstI site found at 6829
  Illegal PstI site found at 8619
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 1946
  Illegal PstI site found at 4982
  Illegal PstI site found at 6829
  Illegal PstI site found at 8619
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 7736
  Illegal BglII site found at 8074
  Illegal BamHI site found at 86
  Illegal BamHI site found at 2451
  Illegal XhoI site found at 595
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 6555
  Illegal PstI site found at 1946
  Illegal PstI site found at 4982
  Illegal PstI site found at 6829
  Illegal PstI site found at 8619
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 6555
  Illegal PstI site found at 1946
  Illegal PstI site found at 4982
  Illegal PstI site found at 6829
  Illegal PstI site found at 8619
  Illegal NgoMIV site found at 10394
  Illegal AgeI site found at 7521
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 9309
  Illegal BsaI site found at 9948
  Illegal SapI.rc site found at 9156
  

Design Notes

The yeast S. cerevisiea does not have a RISC system, which is necessary to use the RNA interference methodology. This cassette was therefore designed with the aim of expressing the RISC system in yeast

Source

BBa_K124002 came from iGEM08_Brown team, responsible for encoding the constitutive promoter of the enzyme glyceraldehyde 3-phosphage dehydrogenase. BBa_K1993016 came from iGEM16_SYSU-MEDICINE and is responsible for coding IRES. BBa_K2637017 came from iGEM18_Tianjin is responsible for coding CYC 1 terminator This sequence was optimized for S. cerevesiae, and the other parts can be obtained from the yeast genome